Cytokinesis looks different in all creatures yet it is always beautifulů

Escherichia coli
(Bacteria)
Expressing FtsZ-GFP in addition to its native copy, undergoing division on nutrient agar on a microscope slide.  To visualize the cell surface, it has been stained with FM4-64, a red fluorescent vital membrane dye.
Sun, Q. and Margolin, W. (1998) FtsZ dynamics during the division cycle of live Escherichia coli cells. The Journal of Bacteriology, 180: 2050-2056.

 

Saccharomyces cerevisiae
(Budding Yeast)
GFP-Myo1p ring and DIC images.
Bi, E., Maddox, P., Lew, D., Salmon, E.D., McMillan, J.,  Yeh, E., and Pringle, J. (1998) Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis. Journal of Cell Biology, Vol. 142(5):1301-1312.

 

Schizosaccharomyces pombe
(Fission Yeast)
The cells are expressing GFP-tubulin as well as GFP-nuclear-pore-protein.
Sorry this movie isn't working. Ask Phong Tran to send us a new one...

by Phong Tran, Valerie Doye, and Fred Chang

 

 

Dictyostelium discoideum
(Slime Mold)
by Noel Gerald and Arturo DeLozanne

 

 

Eyprepocnemis plorans
(Grasshopper)
A short animation of a Meiosis that has been made from images of grasshopper meiosis.
by Miguel Pita

 

Caenorhabditis elegans
(Nematode Worm)
by Ahna R. Skop and John G. White

 

Isolated P1 blastomeres from early C. elegans embryo...
They divide in a line due to asymmetric spindle alignment.
by Ahna R. Skop and John G. White

 

Lytechinus variegatus
(Sea Urchin)
by Julie C. Canman and Mark Terasaki

 

Xenopus laevis
(South African Clawed Frog)
First and second divisions, note the surface contraction waves preceding each division.
by David Gard and Students

 

Surface contraction waves continue in Xenopus embryos treated with the MT inhibitor nocodazole
Indicating the independence of the cytoplasmic clock from the mechanics of mitosis and cytokinesis.
by David Gard and Students

 

Danio rerio
(Zebrafish)
by Leonard D'Amico and Mark Cooper

 

3-D Revolving image of an anaphase spindle
by Aaron Straight

 

Rat kangaroo kidney epithelial cell (PtK1)
Notice both kinectochores of one chromosome were seemingly attached to the same pole at anaphase onset.
by Julie C. Canman, Paul S. Maddox, and E.D. Salmon

 

GFP-Actin in pig kidney epithelial cell (LLPCK1)

by Eric Shelden

 

Tradescantia stamen hair cell
(Spiderwort plant)
by Aline Valster and Peter K. Hepler

oming soon...
Grasshopper Spermatocyte Cytokinesis
D. melanogaster (Fruit Fly) Cytokinesis
and much much more!

Please feel more than free to email pc quicktime movies along with authors names to: jccanman@email.unc.edu

 

Note: The opinions expressed here are those of individual or groups of Cytokinetic Mafia members. The opinions are in no way associated with the Salmon Lab nor with any individual lab or institution where members are employed.

To contact us, email webmaster Julie C. Canman: jccanman@email.unc.edu