- Cut pieces
of double stick tape into 25 x 5 mm strips. Adhere two strips
of tape to the center of a slide; they should be side-by-side, 3 mm
apart and parallel to the long axis of the slide.
- Retrieve a single squeaky
clean coverslip from the storage jar with Dumont #5 forceps.
Dab the edge of the coverslip to remove any large drops of ethanol,
quickly pass it through the flame of a Bunsen burner, and allow the
ethanol to burn off.
- Center the coverslip over
the tape strips on the slide and adhere the coverslip to the strips
of tape, pressing firmly with the back of the forceps to make a good
seal.
- Prepare several perfusion
chambers (~20) and store them in a slide storage box until use.
*=As published in...
Waterman-Storer, C.M. (In press) Microtubule/organelle
motility assays. In: Current Protocols in Cell Biology,
J.S. Bonifacino, M. Dasso, J.B. Harford, J. Lippincott-Schwartz, and
K.M. Yamada, eds. John Wiley, NY.
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