Waterman-Storer, C. M., Worthylake, R.A., Liu, B.P., Burridge, K., and Salmon, E.D. 1999. Microtubule growth activiates Rac1 to promote lamellipodial protrusion in fibroblasts. Nature Cell Biology. 1:45-50.

Movies:

Time lapse multimode imaging of microtubule and cellular dynamics during the induction of microtubule polymerization and activation of lamellipodial protrusion by nocodazole washout in a Swiss 3t3 fibroblast.

Time lapse multimode imaging of microtubule and cellular dynamics during the induction of microtubule polymerization and activation of lamellipodial protrusion by exchange from nocodazol- into taxol-containing media in an NIH-3t3 fibroblast.

Time lapse fluorescence imaging of actin dynamics during the activation of lamellipodial protrusion by nocodazole washout in a Swiss 3t3 fibroblast.

Activation of lamellipodial protrusion by nocodazole washout is inhibited by dominant negative Rac1.