Analysis of Two Component Signaling Systems in Arabidopsis

Analysis of Two-Component Signaling Networks
in Arabidopsis

Joe Kieber University of North Carolina, Dept. of Biology , Chapel Hill, NC 27599
Eric Schaller Dartmouth College, Dept. of Biological Sciences , Hanover, NH 03755
Dennis Mathews University of New Hampshire, Dept. of Plant Biology , Durham, NH 03824

Contact: Joe Kieber | Eric Schaller | Dennis Mathews

An NSF-funded 2010 project focused on understanding the function of the two-component signaling network in Arabidopsis

Two-component systems are the primary means by which bacteria sense and respond to environmental stimuli. These systems are comprised of a number of distinct elements, namely histidine kinases, response regulators and in the case of phosphorelays, histidine phosphotransfer proteins (HPts). Genetic and molecular studies in Arabidopsis have implicated two-component elements in hormone signaling, red-light perception, circadian rhythms and perhaps osmosensing. This site describes studies focused on these Arabidopsis two-component elements.

Abstract of 2010 proposal
Whole genome array of cytokinin-response in Arabidopsis (downloadable data)
Cartoon of two-component signaling
Roles for two-component elements
Publications from 2010 award
Knockout lines for: type-A ARRs | typeB-ARRs | AHPs | AHKs
Promoter GUS fusions for: type-A ARRs | typeB-ARRs
Tables of Arabidopsis two-component elements:
Histidine Kinases | Histidine Phosphotransfer proteins (AHPs) | Response Regulators (ARRs)
Table of cytokinin-regulated transcription factors
Phylogentic trees of Arabidopsis two-component elements:
Histidine Kinases | Histidine Phosphotransfer proteins (AHPs) | Response Regulators (ARRs)
Lineup of Protein Sequences:

Histidine Phosphotransfer proteins (AHPs) | Type-A ARRs | Type-B ARRs ashhhhhrdshdfsh
Reviews on: two-component signaling, ethylene and cytokinins
Other sites of interest: TAIR | PlantsP | Kieber Lab Home page

The left photo shows the phenotype of etilolated Arabidopsis seedlings grown in air (left seedling) or ethylene (right). The seedling grown in the presence of ethylene adopts a morphology known as the triple response. The receptors for ethylene belong to the histidine kinase family. The right photo shows transgenic seedlings harboring an ARR5 promoter-GFP fusion grown on media without (left seedling) or with cytokinin (right seedling) visualized under UV light.

Some material within these Web pages is based on work supported by the National Science Foundation and the United States Department of Agriculture. Any opinions, findings, and conclusions expressed in this material are those of the author and do not necessarily reflect thoseof the supporting agencies.