Imaging
microtubules and microtubule
tips in C. elegans embryos by spinning-disk confocal microscopy
Embryos
from a strain expressing
an alpha-tubulin::GFP fusion protein (gift of K. Oegema) were
visualized
by spinning-disk confocal microscopy. This allowed the visualization of
0.7 µm-thick optical sections and yielded well-resolved,
individual
microtubules. In this movie, both mid-plane imaging (left panel) and
CIMS
(right panel) are shown in the same embryo. The movie starts before
pronuclear
envelope breakdown and ends with cytokinetic furrow ingression.
Anterior
is at the bottom and posterior is at the top. Frames were captured
every
5 sec and are played back at 12 frames/sec.
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