genetic map fragment size cytological location name Colson1999 Civetta2002 forward primer reverse primer annealing temperature MgCl2 concentration (in mM) restiction enzyme different simulans and sech strains (Colson99) sim132 versus sec S-9 (Macdonald99) sim f nt pm st e versus a D. sechellia strain given by J. David (Gleason) sim2 versus sechellia 14021-0248.4 (Civetta) sim f nt pm st e strain versus sechellia 14021-0248.7 (Orgogozo) to order markers Gleason2004 reference 01B1-B14 AF047180 0 taccttaggaaacccgaccc tcttgttgcgaattttgttca 55 2.5 none 248Ð250 "size difference, A" 1 1B3-1B3 scute cactcacttcgagttccctacc ctaaaccctcgcgttatgtacc 58 1.5 none "141, sec>sim, 2%" 1.005 G 2D6 Pgd aggtcatgctgctggtcaa agtcgtcgaccgcacttc 58 2.1 RsaI "1365, RsaI cuts sim only, 2%" 1.01 G 3B2 period cccgtccacgagggcagcgggggc ttctccatctcgtcgttgtg 56 1.5 none "350, sim>sec, 2%" "sim:380 se:350, 2%, 10cm " 1.02 G 3C2 white 3.6 cacatacacagatttattgagccc acacacacttttatactctctccg 53 or 55 1.25 or 2.5 none size difference 1.1 4F1-2 Cdk7 8.9 tatttcgctaacaaaccggc aacgcgatcacaaacatcaa 53 or 55 1.25 or 2.5 none size difference 1.4 04F1-F2 dmu566661 9 tatttcgctaacaaaccggc aacgcgatcacaaacatcaa 55 2.5 none 230Ð250 "size difference, A" no size difference 2 5D5 rux 11.2 CATACCCCTGGAAGAGCAG GATTGTCTGTTGTCGTTTCC 55 1.25 or 2.5 none size difference no size difference 2.1 7C3 dec-1 14.3 atccaatgatgatgcagc caaaggcaatggacagag 53 or 55 1.25 or 2.5 none size difference 2.18 7C3 dec1 gaagatcaggccaaggttca ttctctgccatctgtcgttg 60 1.4 DdeI "300, DdeI cuts sim only (as predicted), 2%" 2.2 G 7F1-7F1 ovarian tumor (otu) tcacgaaatcaaagaaccacc gtctctagccgaacagcgag 58 1.7 SspI "135, SspI cuts sim only" 2.21 G 07F1 drootua x TCACGAAATCAAAGAACCACC GTCTCTAGCCGAACAGCGAG 55 2.5 none 139Ð144 3 08B5-B8 ds09021 G00630 17.7 gatcttttcatgtgttattt ccgttttgtttggcaacttt 55 2.5 none 122Ð124 "size difference, A" 4 9A1-A2 DS01391 gcctgctgcagtcgcatgtg ccagcggcatacgtgtaaac 62 2.0 none ", sec>sim, 2%" 4.01 G 10A1-A2 sev 23.7 catcttaatgaggataaatttgttat aagcgacaagtttcaattaac 53 or 55 1.25 or 2.5 none size difference 4.98 10A1-A2 drosev2 sev 24.4 attaaagtgcaattaactat CATGGCCAATAAGTCACGC 55 2.5 none 236Ð274 "size difference, A" 5 10A1-A2 sevenless attaaagtgcaattaactat aagcgacaagtttcaattaac 52 2.0 none "se:230 sim:275, 2% 8cm" 5.5 11A6-A9 dmtena 28.4 CTCTTAGTGCGCAGGGATTC tcaagagtcgctcaatggc. 55 2.5 none 90Ð98 "size difference, A" 6 13D dmu18774 33.9 gatccttggcggcaggggagcgaa tatgcaactccttgcacaa 55 2.5 none 167Ð200 "size difference, 2%" 7 13E3-8 sog 42.7 gatccttggcggcaggggagcgaa tatgcaactccttgcacaa 53 or 55 1.25 or 2.5 none size difference 7.1 14C1-14C1 nonA gagtatgcacgctatgagca gttgtcaacttcgcgttttc 52 2.8 or 2.0 none "642, sec>>sim, 2%" "sim:430 se:580, 2% 2-3cm" 7.11 G 16E4-F1 Sh 49 caagagatcccgagagagaga acgtgtgcgtgttgtttctc 53 or 55 1.25 or 2.5 none size difference 7.2 15F dmariadne x aacactgtccccatccacat tctgttcaactccttcggct 55 2.5 none 102Ð110 8 16F3-F6 dmtroponi 41.4 CAAGAGATCCCGAGAGAGAGA acgtgtgcgtgttgtttctc 55 2.5 none 110Ð114 "size difference, A" 9 18E Zw ggtgactgtcaacatcaagca tgatcctcgttgaacagacc 54 2.0 none "223, sec>sim, 4M" 9.01 G 19E DROPASSOV 50.7 gtggaaatggcagaggagag gttgttcatttgtttagcgg 55 2.5 none 253Ð263 "size difference, 2%" "sim:250 se:280, 2% 17cm" 10 19E3 shakB 57.9 gtggaaatggcagaggagag gttgttcatttgtttagcgg 53 or 55 1.25 or 2.5 none size difference 10.1 19F3-F6 af017777 x attagctaactccaagaacg aatcctctcagctcagcgta 55 2.5 none 111Ð119 11 21B7-C2 ac006472 x tcctccatgtaaagataaacgct aactcgcaaattgcctaacg 55 2.5 none 169Ð176 12 21C3-21C4 droexpand ex 0 gtgatcgatcccgctgtc tccggtttccaattagcttg 52 2.5 none 80Ð83 "size difference, A" "98, sec>sim, A" 13 G 22D1-22D2 droyanetsb yan 4.1 taatggggaatgggtgaatg gccgtgctcttttctcttacg 54 1.5 none 80Ð88 "size difference, A" "107, sec>sim, 4M" 4M 14 G 22C3-D1 aop 13 taatggggaatgggtgaatg gccgtgctcttttctcttacg 53 or 55 1.25 or 2.5 none size difference 14.1 22F1-22F2 dpp gagctcgactcggtcaacat cattgcgatgagtgggaata 56 1.8 none "609, sec>sim, 4M" 14.11 G 24A1-24A3 odd atgcgacacatggcccacta ttgcggatccagtggttcat 56 2.2 none "188, sim>sec, 4M" 14.12 G 45E1-46A2 ac006472 x tcctccatgtaaagataaacgct aactcgcaaattgcctaacg 55 2.5 none 268Ð270 15 24A1-A2 ds01340 x ggagcgcaatgctgtttaagt ggagtagtgcctgtctcggac 55 2.5 none 160Ð162 16 24C3-D2 ac005732 9.5 taatttggcacaaaccacc. gccgcataatggtcaaaagt 55 2.5 none 208Ð254 "size difference, 2%" 17 24F1-F2 ac004373 x aatgcgtgtgtttggatgaa gtcccagtctcccagtgaaa 55 2.5 none 174Ð178 18 25F5 drogpdha 21.6 cattggaaaagtgagcggat ttggtttgcactccacacat 55 2.5 none 176Ð179 "size difference, A" 19 26A1-26A5 Mst26Aa aacaacccagctaccgatgt ggcckaagcgttatgaatcg 56 1.7 none "206, sec>sim, 2%" 19.01 G 26A5 Acp26Ab 22 atgaactacttcgcggtg atagggttctcaacatgc 53 or 55 1.25 or 2.5 yes restriction enzyme 19.1 28A1-28A3 ninaC 28.5 tttgtcaatctctcacagcagg gccccgagtacatttattcaagc 53 or 55 1.25 or 2.5 none 19.18 28A1-28A3 ninaC gaagtccatcttccaggtcc tatgggcactggcagtggtc 56 2.0 CfoI "261, Hsp92II cuts sim only, 2%" size difference 19.2 G 26A1-C1 ac004758 x tgctttcgctttcggtatct aacggagtgcctatgcatt 55 2.5 none 191Ð203 20 27A1-B2 ac003052 x atcgtcgaacgagaccgta tcgatttaattgcggtgtga 55 2.5 none 239Ð286 no PCR product 21 29A1-C1 AC005555 31.4 ggttgctgggagaaagac gccacacattcgcatctc 55 2.5 none 166Ð178 "size difference, A" 22 30F1-30F6 bib tcgcaaggatcagcggtgac ttgggcctcagcggcagcatt 56 2.0 none "157, sec>sim, A" 22.01 G 30F dmbigene bib x TCGCAAGGATCAGCGGTGAC TTGGGCCTCAGCGGCAGCAT 55 2.5 none 131Ð137 23 31D11-E1 da 47.8 tgcccagcatcacatgatac ggtttttatggaagagaggg 53 or 55 1.25 or 2.5 none size difference 23.8 31E1-E7 drodans x tgcccagcatcacatgatac ggtttttatggaagagaggg 55 2.5 none 273Ð281 24 33A1 sal gccacgatgaaactactggtgc actcctccctggccaattc 58 2.2 none "260, sim>sec, 2%" "sim:290 se:230, 2% 8cM" 24.81 G 33E9-E10 g410 44.5 cfHarr1998 55 2.5 none 125Ð133 "size difference, A" 25 35B2 Adh agcagaccggctaacgagta ggaagagggctccgttagtt 54 1.8 SpeI "695, SpeI sec, SnaBI sim, SpeI is cheaper" 25.01 G 35B10-35B10 Su(H) 64 aacggctcacccctcgatcc tacttctccatggcgtcccg 56 1.9 none "260, sim>sec, minimize amount on gel, 4M" size difference 25.1 G 34C4-D2 ac006302 x tgttttccatgccagctagt gcccggaaaattcttgttta 55 2.5 none 218Ð250 no PCR product 26 35B2-B3 ac004118 x ccaacttgggcgagagaatt gcttaattgcctcactgtgc 55 2.5 none 267Ð270 27 36B1 dromhc x AAACCCACACAACAACTGCA acattaccgatattggatgca 55 2.5 none 90Ð98 28 36C drodorsal 54.3 CTGATGTCGCCCAACCATCC CCGCCAGGATTGCCAAAGGG 55 2.5 none 210Ð228 "size difference, A" no size difference 29 37A1 faf Corbin 61 "SalI cuts se, 2%" 29.5 38D1-D2 ac002474 x gatgctgtccttcggacttc aacaacaaagcccattctgc 55 2.5 none 255Ð258 30 38E1-E9 ac004759 64 acagacggaaagccaaaatg cactccgcctcgtttcttac 55 2.5 none 231Ð237 "size difference, A" 31 38E5-38E6 cad 71.9 tcgggctcggaaatctctag aagtatggcggcttcgatgg 53 or 55 1.25 or 2.5 none size difference 31.08 38E5-38E6 cad cacttggcacaccatttgag gggcttcttcatccagtcga 54 3.0 none "143, sec>sim, 2%" 31.1 G 44F9-45A2 Pgi cctggagcattccaaggag tcggtgaccttttccttgtt 52 1.6 TaqI "421, TaqI cuts sim smaller, 2%" 31.11 G 46E2-E4 DM1433 x gcttgaagaatccctgcttg tgatttttgccagttcagc. 55 2.5 none 101Ð162 no size difference 32 47A drogpad 72.8 AAACCCACACAACAACTGCA AAACCCACACAACAACTGCA 55 2.5 none 150Ð166 "size difference, A" 33 47A1-47A16 Ga47A same as drogpad? gaaataggaatcattttgaatggc aattaaaaacaaaaaacctgagcg 54 3.0 none "178, sim>sec, 2%" "sim:180 se:155, 2% 12cm" 33.01 G 49F9-F13 dmmp20 80.8 CATGCAAATGAGCAGTACTTTG TATTTTCACACATTTCCAATCG 55 2.5 none 76Ð86 "size difference, A" 34 50D1-50D1 mam 100.5 ggcggcctaccagttttcca cctgttgctcccaggtttgc 60 2.0 none "278, sim>sec, 4M" size difference "sim: 300, 2% 10cm" 34.1 G 51A1-A2 dmu19731 x acctttttctcgcagtgcat attgtggctggctgtttacc 55 2.5 none 172Ð178 35 51B6 tra2 CGTTCGCGTTCCTCCTCGG GCCCCGGGAACGCTGTGT 55 2.0 Sau3AI "sim :420+50 se:450+50+70 se3588:350+50 ectopic band at 280bp in both se and sim, 1%" 35.5 51E9-F2 AC004306 x gagacaccccttgacgagtg ctcaaaacaaacccagtctc 55 2.5 none 142Ð146 36 51F11-12 SRPK CAGAGGGAGGAGCGCAAG AGTTGTGGCGGCTGTTGCCC 55 "sim: 350 se:358, 2% 12cm" 36.5 52D2-D15 ac004516 x tcgtcgcccgttaatata accgttcgtgggtcaaatag 55 2.5 none 238Ð276 "sim:295 se:250, 2% 5cm" 37 52D9-D15 AC004248 named sli by Gleason but is not the sli gene 89.3 caatttccctcgcactgacac cggaaacgaacgggcgataag 58 1.4 none 133Ð159 "size difference, A" "150, sec>sim, 2%" se3588:250 sim:150 38 G 53C7 syn2 CATCCCAATGGACCACCCMTGG TTTGTGCTMGGCGCCGCCTT 55 2.0 XmnI "sim:164 se:80+80, 2% 1cm" 38.005 53F13-54A2 Amy-d 115.3 tacgtggatgtgatcttcaa gatgacctcctggacgat 53 or 55 1.25 or 2.5 none size difference "sim:403 se:380, 2% 5cm" 38.1 53D1-E2 acoo4641 x tcaagtagggggtgtcgttc aaccaacaactaattgcggc 55 2.5 none 234Ð236 39 54B1-B2 ds00361 93.1 cfSchlotterer1997 55 2.5 none 142Ð156 "size difference, A" 40 55A1-55C1 grh tccacgacaacgatctcgca ctaacaatgtcgccgggatg 58 1.9 none "113, sim>sec, 2%" 4.5M 3cm 40.01 G 55A1 dmelf1 x tgtaagcaaaggcccagagag gagtctggagctgtaactgaa 55 2.5 none 165Ð186 41 54F1Ð55A1 ac004640 x ccgtaagcccataagcgtaa ggctacggctagagttcgtg 55 2.5 none 242Ð246 42 57C5-D1 ds08687a 102.4 cfSchlotterer 55 2.5 none 173Ð189 "size difference, A" 43 57E1-E2 ac004564 x gaatttcaaaatgggcgaaa attcacgtgctatgtgcagc 55 2.5 none 106Ð113 44 58A4-B1 ac004365 107.6 146.5 gctttatcaatgcagcctcc ggccccaatatgtcctcgcc 55 2.5 none 204Ð210 "size difference, A" size difference 45 59A1-B2 ds08011 111.9 cfHarr1998 55 2.5 none 101Ð111 "size difference, A" 46 59C3-59D2 twist gtgttcctgcccctctacaa cgagctggagtaagcactgc 56 2.0 TaqI "168, TaqI cuts sim only, 2%" "TaqI cuts sim only, sim:120+60 se:180, 2% 3cm" 46.01 G 46.015 46.016 46.017 59A1-D8 dm92d10s x ccgaatcgatgtagttccttg aaggctccggtccttgttag 55 2.5 none 104Ð122 47 62A dmrhob 0 taggcgttcatgttgctcgt taggcgttcatgttgctcgt 55 2.5 none 198Ð206 "size difference, A" 48 62A1-62A3 veinlet tatactaaagtcacttaatgcgttaca gtttgtgtgaatttcactaaattatta 46 2.5 none "195, sim>sec, A" 48.01 G 62B11 Cdc37 0.2 8.8 tatcctatgcaaacacaggcc ggccataactgaaaagctatgc 54 1.6 Alw26I "351, no size diff 4%, Alw26I cuts sim, 2%" size difference 48.1 G 62A1-A2 ac004343 x acggtaattgcggatgagac acgatggcaacaaggatctc 55 2.5 none 202Ð212 "sim:260 se:240, 2% 12cm" 49 63D2-E1 ac004658 6.5 atttggtccacgagagattt tgggaaaactgtgccacata 55 2.5 none 112Ð126 "size difference, A" 50 64B2-64B2 tipE aacgacagttgtctggaggg gcccaggaggagttgagatac 56 2.1 none "269, sim>sec, 2%" "sim:490 se:475, 2% 17cm" 50.01 G 63F1 Acp63F 11.9 CTTGCGTAGAAACTCACTCAGA GTTTTACTTTTACATCCCCTTTAG 45 yes restriction enzyme 50.1 64A dmu36477 x cggcgagccaaactcttat attatttgtggcaaaagcgg 55 2.5 none 103Ð107 51 64B drodsrc 16.5 taggcgttcatgttgctcgt taggcgttcatgttgctcgt 55 2.5 none 312Ð317 "size difference, A" 52 65C3 ple 20.3 ttctgagaggggctttta gaggctaacaaatgaag 53 or 55 1.25 or 2.5 none size difference 52.1 65C5-D1 ac004352 x tcctcggtgagaccgtaatc gggcagaggaaaagcactca 55 2.5 none 267Ð283 53 65D1-D3 dmu14395 28.3 gggcagaggaaaagcactc. tcggtgagaccgtaatctgc 55 2.5 none 263Ð281 "size difference, A" no size difference 54 67B10 Lanb2 30.8 cgtaggaaggaaagaaatcgg aatttgcagttgataggcagc 53 or 55 1.25 or 2.5 none size difference 54.1 67C drolamb2a LamB2 37.9 cgtaggaaggaaagaaatcg ttgcagttgataggcagcat 52 1.5 MfeI 150Ð207 "~200, MfeI cuts sec only" no size difference 55 G 68A8-68A9 Sod cggttttcttcgaacaggag acctcaccggagaccttca 56 2.0 HaeIII "785, DdeI, HaeIII and TaqI all produce diff, HaeIII cuts sim, 2%" 55.01 G 69A1 Est6 cacagaggatggtggctaca ccggacgcatttccacttct 58 1.9 BsrGI "496, BsrGI for sec, BbsI for sim, BsrGI is cheaper" "BsrGI cuts se, 2uL PCR reaction+ 1uL buffer+ 0.1uL BSA + 0.5uL BsrGI+ 6.4uL water, sim:510 se:470, 2% 4cm" 55.02 G 70A Acp70A cgargtatccaattccaagc ttttaagacattggtgtgggttt 52 1.5 none "202, 6bp deletion in sec, sim>sec, 4M" 55.03 G 67D2-D3 drogtpaap x ctgaaatacggcagcagac. taggcgttcatgttgctcgt 55 2.5 none 220Ð226 56 71C-D dmz60mex x AAATCTGTTGCTCATACTGCCC AACCGGCGAAATGTTCAG 55 2.5 none 85 57 73A1-B7 dm22f11t 55.2 56.7 ggatgctcggataccaaaaa tcgcctgtgacttagattgc 55 2.5 none 185Ð203 "size difference, 2%" size difference 58 73A9 tra atttccagcattttccgatg acgtggtagccgttggtatc 50 2.2 none "772, sec>sim, 2%" "sim:700 se:1000, 2% 3cm" 58.01 G 75D1-75E1 dmcathpo cat 60.4 71.7 ttcgacggatcagacttggtttttggc gcgttcgcctttcttagtcaatttcgg 62 2.8 none 108Ð117 "size difference, A" "130, sim>sec, A" size difference "sim>se, 4M" 59 G 76A acp Corbin 55 2.0 BanI 2% 5cm 59.5 77B3 snap GGAAASTTCCTCAGTTCGCTG TCCTCCACRTTGTTGGACCC 52 2.0 ApoI without digest:sim:290 se:276 (ectopic band at 250 in sim sometimes) with digest: sim:250+50 se:135+115+50 (ectopic band in sim cut at 180+50) 59.6 77EE4 kni CTTCACCTGCGAGGGCTGCA GCCGATGGCCTGTTCCTCGA 52 2.0 XmnI "sim:132, se:140, 4M , XmnI cuts sim 70+70, 2% 3cm" 59.7 21Gb ATATCGAAACGAGCGCCCGAT GCGCAGTTCTCTCGTTAATGCTT 52 2.0 HindII "sim:210 se:80+130, 2% 2cm" 59.8 CG1445- ACTCAATCAATTGGGGGCCACT GTGATCGAAATGCGGGGAACAA 48 2.0 Sau3AI "sim:215 se: 100+100, 2% 4cm" 59.85 82A1 CG32490 GGCCGCCAGAGGAATACGA CGGGTGCCACAGCAAACAAGA 52 2.0 none "sim:120 se:110, 2% 10cm" 59.9 82B rpk agccggcgatgacttggactacttt agcgcttttactcttttgaattccctgact 55 2.0 PVUII 1% 2cm 59.95 84C1-C4 AC001665 134.6 atgctacactcaaacaaatggg ttggaaccaaggatcttactgc 53 or 55 1.25 or 2.5 none size difference 59.98 84C1-C4 AC001665 dro17dc4z 69.4 tacatgtgctgcatctgttgc tacatgtgctgcatctgttgc 55 2.5 none 134Ð140 60 84D1 Gld acgtcttagccgaagtcagg cgaactcaacgaaagayagc 54 1.6 HaeIII "251, HaeIII cuts sim and not sec, 2%" 60.01 G 84D3 rn CGCCCCACGATGCAGAACAAAC ACCCGCTGCCAAGTGACGACATT 60 2.0 AluI "AluI cuts 3x se and 2x sim - 2 main bands (500 and 200) for sim, 2 main bands (350 and 200) for se, 3 main bands (500, 350 and 200) for F1, 2% 3.5cm" 60.5 93E9-F1 e2F CGCTCTTCAACAATATCGATGC GCAAGAACTCAGACTGTGATG 55 2.0 HincII "sim: 1400 se:700+700, 2% 2cm" 60.6 93E4 InR CCTTATAGCTTGCAAATCCATGG GACCAAGGCATATCCGTCGA 52 2.0 HindIII "sim:250 se:120, 2% 4cm" 60.7 93D9 tin GGTATGCCCGCCCACATGTT TAGCGGCGATTCTGGAACCA 52 2.0 none "sim:1150 se:1020, 0.8% 8cm" 60.8 93D4 hsr-omega GGGCAAGGGCCCACGTAGT CGATATCAACTTTCAACGCCCA 52 2.0 DdeI "sim: 500+250+180+100 se:600+250+180, 1% 4cm" 60.9 93D2 CG3308 CCCCAGGAGACGGTGGTGG TGCACGGCTCGTTGCGTTGG 55 2.0 HincII "sim:1450 se:400+1050, 1% 4cm" 60.95 CG5892 CTGCCCCATTTGGGCGACGG GAGCTGGTCTTCGGTTCGGCA 55 2.0 ClaI "sim: 800+140+50 se: 800+190 ectopic band at 450bp, 2% 4cm" 60.955 92F3 Hairless ACGCCTTCAGAGCGCATTGG GTCGCCGATGATGGTGGAGAA 52 2.0 none "sim:240 se:210, 2% 6cm" 60.956 3'Dl CGACAGGCCGGCAATGGG TTGAATGCGGACAAAAGAACCCG 52 2.0 AluI "sim:240 se:110+130, 2% 4cm " 60.957 91E1-E3 drohoxnk4 x ctgaagttgaagtccgagcc tacatgtgctgcatctgttgc 55 2.5 none 136Ð139 61 91F13-91F13 dronanos 77.8 cgcaagtattcatttcaacaca tgctggcggttgtttcat 54 1.8 none 104Ð110 "size difference, A" "130, sim>sec, 4M" 62 G 91F13-91F13 nanos CAAGCGAACAACAGCAAGGC CCCTATCGGCCACGACGATT 52 2.0 XmnI "sim:704 se:320+380, 2% 4cm" 62.005 91A1-91A2 glass gcaccattgcacagatatgc tcttccagggaatctgcg 52 2.0 or 2.5 RsaI "285, RsaI cuts sec only, 2%" "RsaI cuts se, sim:280 se:220" 62.01 G 89E1-89E1 Fas1 gatgattcggaactctctcagg tttgcaccaactgtttctgc 56 2.5 none "194, TaqI cuts sim and not sec, 4M" 62.02 G 90C10-D1 cpo 97 tcgcacgagtccaactcc acggagtccatgctctgc 53 or 55 1.25 or 2.5 none size difference "sim:280 se:250, 2% 5cm" 62.1 90B1-B2 dmehab 84.4 atttattagttttttgctaatacttgc agagttcttgttgtatttatac. 55 2.5 none 353Ð361 "size difference, A" 63 89A1-A5 AC006414 94.7 gaaagagctccaaggcaatcagg tgtttcccaggacaggataagcg 52 or 55 2.5 none 192Ð198 "size difference, A" "sim:98 se:92, 4M" 64 88F5 drotropi2 x gtacatcccgaatcccacac aatacactgaaactgttggggg 60 2.5 none 84 65 88B3 dmtrxiii 100.3 gaccgtttgtttgccttgat tgcctgtacaagtctgaccg 55 2.5 none 211Ð214 "size difference, A" 66 86E3-86E3 dmprosper 107.1 caataaccacacgcattcc. aaccacttcctgtttggcc. 55 2.5 none 110Ð114 "size difference, A" 66.98 86E3-86E3 pros cggtacaaagtgtgtgttc gacttttaaacatttaagattaattcc 48 2.0 CfoI "205, CfoI cuts sim only, 2%" "HinPI1 cuts,205, 2% 4-5cm " 67 G 85E10-85E13 Mtn aactcaatcaagatgccttgc ttgcagtcagatccgcagt 52 1.8 DraI "319, DraI cuts sec only, Tru9I produces different patterns, 2%" "ApoI cuts sim, without digest:sim:319 se:325, 2% 3cm; with digest: sim:250+70 , se:325, 2% 10cm" 67.01 G 84E12-13 CG7891 CCGGGAAGACGACGTTYGTC GCCTCTGGTGATCTTGCGCAT 52 2.0 BrsBI "BrsBI cuts se ,sim: 1300 se:1150+150, 2% 5cm" 67.05 94E11-F1 pnt 141.5 caataacaattgccacacgg aattggtgatgcggatgg 53 or 55 1.25 or 2.5 none size difference 67.1 93F dmu25686 x cgataatttactctgtgctcc cagctcacacaaaaggcaaa 55 2.5 none 153Ð155 68 95C6-C8 dmtf125 119.7 ctcgagcgggccatacaaga tgattgaagaggccactcaa 55 2.5 none 289Ð297 "size difference, A" no size difference 69 96A17-96A17 slo gatgctgccaacatcatgag cgacgtcttgaacgatctca 54 2.5 none "248, sec>sim, 2%" "sim:320 se:340, 2% 12cm" 69.01 G 98B1-98B8 Mlc1 ccgaggatgatgaaggattt cgggtctgtcacacattctg 58 1.5 HinfI "571, HinfI cuts sec only, 2%" 69.02 G 99D4-99D8 jan cgcattgaacacaatcccga ctcatcggagatttcgatcg 54 2.0 DdeI "243, DdeI cuts sec only, 2%" 69.03 G 99D6-99D9 sima tgcacccagcaataccagta gctgttgtcgtggtgctg 60 1.5 Hsp92II "187, Hsp92II cuts sec only, 2%" 2% 7cm - good to distinguish se from F1 but not sim from F1 69.04 G 100D-100E eF1 gctgtgcgtgacatgagg tgctgaaacatcatcttatcgg 54 1.5 none "308, sec>sim, A" 69.05 G 99D5 Anp 173.6 tgcttctcagtctaaatcat GTATTGCGTTTTCCTGTAG 55 yes restriction enzyme 69.05 96E1-E4 dmsidna x gctgttgtcgtggtgctg gctgttgtcgtggtgctg 55 2.5 none 126Ð128 70 99D6-D9 DMU43090 132.8 tgcacccagcaataccagta gctgttgtcgtggtgctg 55 2.5 none 180Ð186 "size difference, A" 71 ci GCGAGTATCCGGGATGTAGC CCGTTGCCTAGCCAAAACAG 55 2 SacI "SacI cuts se, sim: 600 sech:450+150, 2% 3cm" 75 2% agarose 4% Metaphor agarose Acrylamide